Hello,
I have quite a simple question.
I have a huge file of 7 GB (approx. 43 million sequences). I want to blast my 1 sequence (1000 bp) against it. Is it possible to do it on a normal computer with 300 GB free space? I get result of only 97 hits found, but I guess that blast may be interrupted due to space or power limit. Also, I tried to divide them in smaller files, but my computer simple gets stuck. Any advice or comment is appreciated.
