Bioinformatics noob here,
Im working with a firefly database. I have downloaded the blast executables and made a local database with the makeblastdb command. To test the database I have used the blastn command using a luciferase gene as the query, which I know is in the database because I have blasted before in a different program, and I get **No hits found**. I have tried this with several different luciferase sequences and each time I am getting **No hits found**.
Does anyone have any idea as to why this is happening? Do I have to change any search parameters?
