Quantcast
Viewing all articles
Browse latest Browse all 41826

Ncbi Blast Output Missing Subject Name

I'm wondering if anyone has stumbled upon this issue I'm having with blast. I have a set of contigs I'm blasting against NCBI's NR blast database, and the blast report I get back has a blank field for the subject for the hits I get.

These are the blast commands I am using:

blastx -subject blastdb/nr.pal -query 0.fa -out 0.blast.out
blastx -db blastdb/nr.pal -query 0.fa -out 0.blast.out

I have ended up with output like this:

BLASTX 2.2.25+


Query= 5350 787 3793

Length=787

Subject=
Length=124


 Score = 13.9 bits (24),  Expect = 1.9
 Identities = 4/6 (67%), Positives = 6/6 (100%), Gaps = 0/6 (0%)
 Frame = +1

Query  763  IPLQSF  780
            +PL+SF
Sbjct  85   MPLESF  90


 Score = 11.9 bits (19),  Expect = 7.3
 Identities = 4/9 (45%), Positives = 6/9 (67%), Gaps = 0/9 (0%)
 Frame = +1

Query  463  PENFV*SSP  489
            P++   SSP
Sbjct  43   PDBSWISSP  51

As you can see the Subject= is empty in these results.

I have tried changing the output to blastxml, I've tried downloading the fasta file for NR, and formatting that into a blast database, and I still end up with the same situation. My question, is there some option I'm missing from blast that omits the subject, or a different way to specify the blastdb?

I'm running NCBI blast 2.2.25+, and using the NR file and databases from NCBI.
Thanks in advance!


Viewing all articles
Browse latest Browse all 41826

Trending Articles