Hallo...how to one examine whether homology method used is feasible for identification of a particular gene family e.g HSP. Suppose i have identified 550 sequences suspected to be a particular gene family and i want to know if actually this are my target genes which approach will suitable?. Given that some previous research has been done before of this family and around 200 was identified then...Will reciprocal BLAST work well?if yes how will i know for instance that some of my identified genes are similar with the genes identified earlier on by other researchers given that the Accesion numbers of my identified sequences are different with the ones identified earlier on?
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