I have a quick question: How can I turn off search on reverse complement strand of my query nucleotide sequence in blastn?
For example, I don't want 'GUAAAGCCAAAUCUUCGGUUA' to be a hit when I use 'UAACCGAAGAUUUGGCUUUAC' as the query.
Maybe I missed it when I read the man page, but I really appreciate it if someone can point out the parameter I should use.
Thanks!