Ncbi Web-Blast With Huge Subject Sequence Provided By The User In Fasta File

hello everyone,

I tried to blast my query sequences with my subject sequences in fasta file...i uploaded both my query and subject sequences in fasta file to the ncbi blast page, but it didnot respond..I used 64 bit computer with 16GB RAM....I couldnot figure out the problem..Can anyone suggest me something....

Thanks!