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run multiple sequences through psipred

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Hi, I have psipred working (thanks to some posters on here) and can confirm it works for single sequences - however when I input a fasta file with multiple sequences it only gives the output for the last sequence in the file. does anyone know how to get round this?

here is the runpsipred.pl script:

#!/bin/tcsh

# This is a simple script which will carry out all of the basic steps
# required to make a PSIPRED V3 prediction using BLAST+. Note that it
# assumes that the following programs are in the appropriate directories:
# psiblast - PSIBLAST executable (from NCBI BLAST+)
# psipred - PSIPRED V3 program
# psipass2 - PSIPRED V3 program
# chkparse - PSIPRED V3 program

# NOTE: Experimental PSIPRED script with BLAST+ compatability (DTJ May 2010)

# The name of the BLAST+ data bank
set dbname = uniref90filt

# Where the NCBI BLAST+ programs have been installed
set ncbidir = /usr/local/bin

# Where the PSIPRED V3 programs have been installed
set execdir = ../bin

# Where the PSIPRED V3 data files have been installed
set datadir = ../data

set basename = $1:r
set rootname = $basename:t

# Generate a "unique" temporary filename root
set hostid = `hostid`
set tmproot = psitmp$$$hostid

\cp -f $1 $tmproot.fasta

echo "Running PSI-BLAST with sequence" $1 "..."

$ncbidir/psiblast -db $dbname -query $tmproot.fasta -inclusion_ethresh 0.001 -out_pssm $tmproot.chk -num_iterations 3 -num_alignments 0 >& $tmproot.blast

if ($status != 0) then
    tail $tmproot.blast
    echo "FATAL: Error whilst running blastpgp - script terminated!"
    exit $status
endif

echo "Predicting secondary structure..."

$execdir/chkparse $tmproot.chk > $tmproot.mtx

if ($status != 0) then
    echo "FATAL: Error whilst running chkparse - script terminated!"
    exit $status
endif

echo Pass1 ...

$execdir/psipred $tmproot.mtx $datadir/weights.dat $datadir/weights.dat2 $datadir/weights.dat3 > $rootname.ss

if ($status != 0) then
    echo "FATAL: Error whilst running psipred - script terminated!"
    exit $status
endif

echo Pass2 ...

$execdir/psipass2 $datadir/weights_p2.dat 1 1.0 1.0 $rootname.ss2 $rootname.ss > $rootname.horiz

if ($status != 0) then
    echo "FATAL: Error whilst running psipass2 - script terminated!"
    exit $status
endif

# Remove temporary files

echo Cleaning up ...
\rm -f $tmproot.* error.log

echo "Final output files:" $rootname.ss2 $rootname.horiz
echo "Finished."

can anyone see why it is not storing (or rather overwriting) n-1 outputs?

if anyone can point me to where i need to change this that would be great.

thanks, arron.


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