Problems In Creating Pssm Using Psiblast

Hi, Everyone.

Recently I am using PSIBlast to create PSSM.

Command seems like this:

psiblast -subject oneseq.fasta -inmsa alignment.fasta -outascii_pssm pssm.txt

The problems is

Error: NCBI C++ Exception: "/am/ncbiapdata/release/blast/src/2.2.25/IntelMAC-universal/c++/GCC401-ReleaseMT--IntelMAC-universal/../src/util/sequtil/sequtilconvertimp.cpp", line 225: Error: ncbi::CSeqConvert_imp::Convert() - Unknown conversion.

I have tried several times and did not figure out this problem.

Could anyone can help me to solve this problem?

Great thanks.