Hi All,
I am using blastn to blast my data against tRNAs which is extracted from RFam database. Then I picked one match from the blast result to confirm it by directly searching my read in RFam online (http://rfam.sanger.ac.uk/search).
Here is my read which have a 100% match with a tRNA: CCCATTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGC
This is the matched tRNA from RFam: GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCCCATTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGCA
Clearly it's a perfect match (in bold). But when you search my query in RFam, there is no hit. However, the subject tRNA can be found in RFam.
Could anybody tell me what's the problem?