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Is Makeblastdb Creat 3 Files Like Formatdb + Blast Error

Hi everyone,

I have a big problem!

I install blast+ for windows.

I run this :

makeblastdb.exe -in filename.fasta -dbtype nucl -out filename_BLASTdb

apparently it create db coz I have this message:

a -dbtype nucl -out 020_GC.LD_ED_19July_BLASTdb


Building a new DB, current time: 07/20/2011 09:47:50
New DB name:   020_GC.LD_ED_19July_BLASTdb
New DB title:  020_GC.LD_ED_19July.fasta
Sequence type: Nucleotide
Keep Linkouts: T
Keep MBits: T
Maximum file size: 1073741824B
Adding sequences from FASTA; added 809 sequences in 0.289397 seconds.

right?

but there is no file created so when i try to blast

blastn.exe     -db filename_BLASTdb -word_size 7 -query mature.fasta -out filename.blastsmallRNA     -evalue 10

there is an error i can not understand:

Error: (106.18)
NCBI C++ Exception:
    Error: (CArgException::eSynopsis) Too many positional arguments (1), the off
ending value: filename.fasta-db
    Error: (CArgException::eSynopsis) Application's initialization failed

could you please help

thanks


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