Hi everyone,
I have a big problem!
I install blast+ for windows.
I run this :
makeblastdb.exe -in filename.fasta -dbtype nucl -out filename_BLASTdb
apparently it create db coz I have this message:
a -dbtype nucl -out 020_GC.LD_ED_19July_BLASTdb
Building a new DB, current time: 07/20/2011 09:47:50
New DB name: 020_GC.LD_ED_19July_BLASTdb
New DB title: 020_GC.LD_ED_19July.fasta
Sequence type: Nucleotide
Keep Linkouts: T
Keep MBits: T
Maximum file size: 1073741824B
Adding sequences from FASTA; added 809 sequences in 0.289397 seconds.
right?
but there is no file created so when i try to blast
blastn.exe -db filename_BLASTdb -word_size 7 -query mature.fasta -out filename.blastsmallRNA -evalue 10
there is an error i can not understand:
Error: (106.18)
NCBI C++ Exception:
Error: (CArgException::eSynopsis) Too many positional arguments (1), the off
ending value: filename.fasta-db
Error: (CArgException::eSynopsis) Application's initialization failed
could you please help
thanks