Hi,
I am trying to run MEGAN5 using a blast table generated by an associated researcher.
I know that MEGAN is able to interpret blast files fomatted by the m8 option. I have tried to load the tables without success, and I believe that it is related to the field order of my tables.
Does anybody know the correct format of tabular tables that I must provide to MEGAN?
Thanks in advance.
Here is a sample of my tables:
# Fields: query
_id(sample
contig
start
end
strand
length)
subject_id
subject_function
subject_organism
%_identity
%_positive
alignment_length
gaps
query_start
query_end
subject_start
subject_end
evalue
bit_score
taxonomy
6
contig00001
1
411
0
136
WP_003253097
DNA_methyltransferase
Geobacillus_thermoglucosidasius
133
136
136
0
1
136
2
137
4.79761e-90
716
Bacteria_Firmicutes_Bacilli_Bacillales_Bacillaceae_Geobacillus
6
contig00001
439
3295
0
949
WP_003354162
DNA_methyltransferase
Bacillus_smithii
585
613
642
0
67
708
1
642
0
3118
Bacteria_Firmicutes_Bacilli_Bacillales_Bacillaceae_Bacillus
6
contig00001
3472
3546
0
23
unknown
unknown
unidentified
0
0
0
0
0
0
0
0
0
0
unidentified
...
↧