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How To Blast Kegg-Only Data In The 'Nr' Database

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Hi there,

I dont have a locally KEGG database bought,but I want to Blast my sequences against only the KEGG part of nr db cause you know blast whole nr is really time consuming.

Here is my approach:

  1. download the KO number of a specific pathway that I'm interested in from the KEGG server
  2. convert the KO number downloaded into GI number
  3. then use the blast+ 's gilist function to Blast the GI numbers that I downloaded.

But MY PROBLEM is: I dont know how to do the step 1 and step 2, could anyone provide some procedure to do that? Many thanks,

Kylie


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