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Reciprocal blast and protein identification

Hi Guys,

I am doing reciprocal BLAST to identify members of a protein family in different species. Is it necessary for me to set a strict e-value threshold while doing each BLAST? What I am doing is I am setting e-value of 1 in each BLAST search. Once I finish doing reciprocal BLAST, I am planning to do the phylogenetic analysis to further confirm the evolutionarily related gene members in other species. Could you please let me know of anything I am missing in my process of identification? 

Thank you.


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