I am planning to set up and maintain a local version of the NR and other NCBI databases, for running in-house BLAST searches. I would also like to my local version of the databases be in synch with NCBI through regular updates. NCBI suggests using the update_blastdb.pl (http://www.ncbi.nlm.nih.gov/blast/docs/update_blastdb.pl) to download the latest versions of all the pre-formatted databases. Does anyone have experiences to share on using this script? Are there alternative solutions? Will appreciate everyone's feedback. Thanks, Anjan
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