The attached file mouseReads.fasta (Copy/pasted below) contains 67 short reads from a mouse RNA-Seq experiment. The reads were downloaded from the NCBI Short Read Archive. Millions of reads were generated in the experiment. The 67 reads are chosen because they cannot be mapped to the mouse genome by BOWTIE. Your task is to use BLAST or BLAT to find out how many reads can be aligned to mouse (either the genome or any mouse sequence) at the E-value cutoff of 0.01 (not the default cutoff), against refseq_RNA and refseq_genomic.
When I tried doing this using BLAST and BLAT, I came across error message stating no significant values found. Any help would be appreciated.
>ERR032221.2_IL9_4848:1:1:1146:7673/2
CGNAGGGACGGGCGACTGGCCCCCCTGGCCCCCGGCGGGCGCAAAGGGGGCGGGGGGCCGNCCCCCCCCCCCGCGG>ERR032221.5_IL9_4848:1:1:1147:9717/2
CTNGTGCTCACAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGTGTACATATGAAAGTGTAANTTCTCTGTATTTTTT>ERR032221.10_IL9_4848:1:1:1149:16450/1
CTCATCACTACCCTTTTGTGTTNTCCTCATTAANCATTCCCGTTAGTCCTCTTGCCGCTAGTGCCTTANACCCAAC>ERR032221.24_IL9_4848:1:1:1152:8320/2
CANGAAGCCAACGTTCCTTTTCTTATTTTTTTTTTTTTTTTTACTTGTCTTCCTCCCCACNCAAAAACAGAAAAGA>ERR032221.25_IL9_4848:1:1:1152:11592/1
CTTAGACATGCATGGCTTAATCNTTGAGACAAGNATATGCTACTGGCAGGAAGAGGAAATTCTCAGCTNCATCGTG>ERR032221.28_IL9_4848:1:1:1153:1705/2
GGNAACTTATAGAGCACCTTCAGGTGACAGGATGACAGGACATCAAGTGAGGAGTGGTGANGGGAGAGGGGTAGGT>ERR032221.30_IL9_4848:1:1:1153:14089/2
GCNTTCCCCCATCCCAACTGGAAGCGAAAAACCAAAACAAAACAAAAATAACTCTGAAGG ...