I created the database of 1 chr using makeblastdb tool of localblast. It showing me output, But it also showing me warning while i am blasting from programming. that ..
[blastall] WARNING: Unable to open bos.db.nin
[blastall] WARNING: Unable to open bos.db.nin
[blastall] WARNING: Unable to open bos.db.nin
[blastall] WARNING: Unable to open bos.db.nin
[blastall] WARNING: Unable to open bos.db.nin
.. is it an error or should i ignore this.bcs it showing me correct blast result.