Quantcast
Viewing all articles
Browse latest Browse all 41826

How to get the result which mismatch 1 to the query sequence by Nucleotide-Nucleotide BLAST 2.2.27+

the program is blastn -task blastn-short -query rfam_trimed_short_uniq.fa -db /root/bio-analysis-soft/database/gss-est-db/tage -ungapped -penalty -1 -reward 1 -outfmt 6 -out 2 -num_threads 12

I get an error.Error: NCBI C++ Exception: "/root/bio-analysis-soft/msw/ncbi-blast-2.2.27+-src/c++/src/corelib/ncbiobj.cpp", line 689: Critical: ncbi::CObject::ThrowNullPointerException() - Attempt to access NULL pointer.

I think the error caused by -num_threads 12.can you help me ?


Viewing all articles
Browse latest Browse all 41826

Trending Articles