I'm new to BLAST and am trying to run a legacy blast command using blast+. I have downloaded the human_genome databases and unzipped and untarred them.
The databases are in: /home/assay/assay/bin/blast/db/human_genomic
They look like this:
human_genomic.01.nsd human_genomic.03.nsd human_genomic.05.nog human_genomic.07.nni human_genomic.09.nnd human_genomic.11.ninhuman_genomic.01.nsi human_genomic.03.nsi human_genomic.05.nsd human_genomic.07.nog human_genomic.09.nni human_genomic.11.nn human_genomic.00.nhd human_genomic.01.nsq human_genomic.03.nsq human_genomic.05.nsi human_genomic.07.nsd human_genomic.09.nog human_genomic.11.nni .... etc ...
There is also a human_genomic.nal file in the directory
The command line I am using & error message is:
$ ./legacy_blast.pl blastall -p blastn -d human_genomic -m 7 -q -2 -W 7 -e 1000 -F T
BLAST Database error: No alias or index file found for nucleotide database [human_genomic] in search path [/home/assay/assay/bin/blast/bin:/home/assay/assay/bin/blast/db:]
Program failed, try executing the command manually.