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Creating A Dna Sequence Database Locally For Blastplus

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Hi,

I have a fasta file containing cDNA sequences and I would like to create a blastable database for blastplus, (using blastn) that I have installed locally. I am, however, unsuccessful up to now as I end up creating a PROTEIN database, although my file obviously contains DNA sequences. Here is the command I use, following blastplus user_manual.pdf:

makeblastdb -in my_file.fasta -title my_db -out ~/path/blastplus/database/my_db

And here is the output produced:

Building a new DB, current time: 07/26/2010 11:00:28
New DB name:   /path/blastplus/database/my_db
New DB title:  my_db
Sequence type: Protein
Keep Linkouts: T
Keep MBits: T
Maximum file size: 1073741824B
Adding sequences from FASTA; added 1100 sequences in 0.0458999 seconds.

The user manual says that makeblastdb is supposed to recognise the data type automatically. Should it not recognize that I'm using DNA and not proteins?

Your suggestions are appreciated :)

Cheers


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