The attached file mouseReads.fasta (Copy/pasted below) contains 67 short reads from a mouse RNA-Seq experiment. The reads were downloaded from the NCBI Short Read Archive. Millions of reads were generated in the experiment. The 67 reads are chosen because they cannot be mapped to the mouse genome by BOWTIE. Your task is to use BLAST or BLAT to find out how many reads can be aligned to mouse (either the genome or any mouse sequence) at the E-value cutoff of 0.01 (not the default cutoff), against refseq_RNA and refseq_genomic.
When I tried doing this using BLAST and BLAT, I came across error message stating no significant values found. Any help would be appreciated.
>ERR032221.2_IL9_4848:1:1:1146:7673/2
CGNAGGGACGGGCGACTGGCCCCCCTGGCCCCCGGCGGGCGCAAAGGGGGCGGGGGGCCGNCCCCCCCCCCCGCGG
>ERR032221.5_IL9_4848:1:1:1147:9717/2
CTNGTGCTCACAGAGAGAGAGAGAGAGAGAGAGAGAGAGGGTGTACATATGAAAGTGTAANTTCTCTGTATTTTTT
>ERR032221.10_IL9_4848:1:1:1149:16450/1
CTCATCACTACCCTTTTGTGTTNTCCTCATTAANCATTCCCGTTAGTCCTCTTGCCGCTAGTGCCTTANACCCAAC
>ERR032221.24_IL9_4848:1:1:1152:8320/2
CANGAAGCCAACGTTCCTTTTCTTATTTTTTTTTTTTTTTTTACTTGTCTTCCTCCCCACNCAAAAACAGAAAAGA
>ERR032221.25_IL9_4848:1:1:1152:11592/1
CTTAGACATGCATGGCTTAATCNTTGAGACAAGNATATGCTACTGGCAGGAAGAGGAAATTCTCAGCTNCATCGTG
>ERR032221.28_IL9_4848:1:1:1153:1705/2
GGNAACTTATAGAGCACCTTCAGGTGACAGGATGACAGGACATCAAGTGAGGAGTGGTGANGGGAGAGGGGTAGGT
>ERR032221.30_IL9_4848:1:1:1153:14089/2
GCNTTCCCCCATCCCAACTGGAAGCGAAAAACCAAAACAAAACAAAAATAACTCTGAAGGNCGGGAGGATACCCAA
>ERR032221.31_IL9_4848:1:1:1154:1425/2
GGNTGAGAGTGTGCACAGACCAGAACTCTGCTCCTCTCATCTGAGGAGACCTCACTCGGANTTACAGACATGAAAG
>ERR032221.42_IL9_4848:1:1:1155:20781/2
TTNGGAGATTNGTTGATGTATGAGGTTGATGATGTTNGAGTTATGTTGGAAGGAGGGACCNGAGATACATTGATGA
>ERR032221.46_IL9_4848:1:1:1157:5641/1
ACAAGCTTTACTGCTCCCACAANCCTGGCTCTCNGGTGGTCAAACTGAAATAGTTGCCCATAGGATAANACATATG
>ERR032221.49_IL9_4848:1:1:1158:7388/2
CTNCTCTCTTCTCACCCAGTTCCGGCAGTGTATCTCTGCGTATGAAACAGGTGGGAGAACTATATCAGTCTCTGCT
>ERR032221.70_IL9_4848:1:1:1161:10074/1
AGGCCACCAAGTTCTGATAAGTNACAAGCCACCNACAAACACACACATAACTATAAATGACAGAGGAGNTCTTAAA
>ERR032221.89_IL9_4848:1:1:1165:3542/1
TCGGGGATTGCAATTATTCCCCNTGAACGAGGCNGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCNGGTTAAT
>ERR032221.94_IL9_4848:1:1:1167:13943/1
GACCAACTCAGACCTGGTCAGCNTTGCCAAAGANGTATCCCAGATGGTAGCCAGGGACATGATTAACCNTGGCGTG
>ERR032221.104_IL9_4848:1:1:1168:12666/2
TANCACTTCATTGTAAAATTTATAAAAAGAACAGTTTCACCCCCCCCCCTCCACCCCCCATTCCACTCCCCACCAC
>ERR032221.106_IL9_4848:1:1:1168:10565/1
TTTGGATCCTATGACATTGATGNGCAGAAGCGGNGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGNAGGAGGT
>ERR032221.109_IL9_4848:1:1:1169:17104/1
TGGGCAAGTTCACATCCACTTCNGGGCTGACATNGATGTCTCAGGACCCAAGGTGGACATTGATGTTCNTGATGTG
>ERR032221.118_IL9_4848:1:1:1171:9299/2
CGNGGTGTCTTTACTGCAATGGGTCAAAAATCAATCTCATCTACTCCCGAGACCATTCTGTGGACGAGAGCCTTGA
>ERR032221.121_IL9_4848:1:1:1172:13465/1
GGTGTCTGTAGATGTGGGAAACNCCTGCTTGGGNGGGGGGGGGCATTTCAGGGCAGCACCAGGGACTTNTCTTTTT
>ERR032221.124_IL9_4848:1:1:1172:17042/2
TCNCCCTGCCCAGGCATAGTTCACCATCTGGGTGACCCTTACATATTACCAAGTCCCACTGCAGCAGGAGTACAAC
>ERR032221.134_IL9_4848:1:1:1174:15810/2
GTNGAAGATTCAGAGGAGATGAAAAAGACCTCTGACCCAAGAAGCTGTGACAGGAGGAGATGGAGATAGAGACAAG
>ERR032221.142_IL9_4848:1:1:1177:13005/2
GCNGTATCAGGATGGCGTATTCAAGTTTACGGTAGGTGAAGAGAAGACATTAGCAGGAGATGTGAAAACCAGTCCT
>ERR032221.167_IL9_4848:1:1:1181:6163/2
CCNAGCTTCATCCTTCTTCATCAGCATCCTAGTACTCAGGAGGCTGAGGCAGGAGGATTGATAGTTTGAGGCTAGC
>ERR032221.170_IL9_4848:1:1:1181:3408/1
GCGTTTCCACATGGAAGGTGCGCTCAATGACTANTATGCAATTGAAGACATTCTCAGTGACATCTCAGNAATTGAT
>ERR032221.177_IL9_4848:1:1:1182:17293/2
TTNCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGCACCTCAGACCAGATGGGTTGGCCTG
>ERR032221.203_IL9_4848:1:1:1188:10846/2
CTTAAATCTCTTTATGAAGATTTTATACCAGGTGTGAAAGAGCCTCCAGAAGCTTCTTCACGGCCTCGGAAGCCTC
>ERR032221.207_IL9_4848:1:1:1188:17315/2
TTGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGCACCTCAGACCAGATGGGTTGGCCTG
>ERR032221.212_IL9_4848:1:1:1189:19230/1
CTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGNGAAAAGTTACCACAGGGATAACTGGCTTGTGGCANCCAAGCG
>ERR032221.215_IL9_4848:1:1:1189:20252/2
CTTGGGGTGGGGCATTGAGAGATGATCAGTGCTGTGGGTCAGTGATTAGTCAGGGGCAACTTAACTTTTAGAAAGA
>ERR032221.220_IL9_4848:1:1:1190:2796/1
AGAGGATCTGTGTTGATGCCTAAGCAATATATANGCACTCTTGGCTGCTGAGCCATCTCTCCAGCCTCNATGTTTG
>ERR032221.225_IL9_4848:1:1:1192:13666/1
TGGCAAGTGAAACTCCACACCTCCCCTTGAGATNGTGGGTGGGGTGAGCTCTCTGAACATAGTTTGATNTTCTTTT
>ERR032221.229_IL9_4848:1:1:1192:8700/1
TGGTGGTGCTACACCCTTTAATCCCAGCACTCGGGAGATATTTTTTTAAAGTAGACTGTATTTGACTTNAGAGTTG
>ERR032221.232_IL9_4848:1:1:1193:11244/1
CTCCGTACTTGAGCGCGATGCCCTGAAGCGTGTCCCCGGCGCGGAGTCACGATGGAACAGATCAAAAGNGCCAACA
>ERR032221.250_IL9_4848:1:1:1196:13511/2
CCCTGCTCCATCCTGGCCACCACTCTCCAAGAATTTCTTAAAACCGTCCAGTGTCCCGCCGTGCGGCTTATTACCC
>ERR032221.254_IL9_4848:1:1:1197:10809/2
CCCCCAAACACCTCCCCCAAAAAACAACTTCCTCTTCTTCGCACATATTTGGGAACACAAAAACTATATTGTGCTA
>ERR032221.271_IL9_4848:1:1:1199:2817/1
AGAGGATCTGTGTTGATGCCTAAGCAATATATATGCACTCTTGGCTGCTGAGCCCTCTCTCCAGCCTCNCTGTTTG
>ERR032221.286_IL9_4848:1:1:1203:1362/2
CCTTACTGTTTAAGGAACATTTGGCTTTTTTTTTTTGTGGGTGAAACACGAAGGAACCCGGCCCCTCCCCCGCTTG
>ERR032221.290_IL9_4848:1:1:1205:2246/2
GGGTAGGAAAGGTCATAAACATTGCACAGAATCATCTAAAGGAAAAGCATCAGCAAGAACTCCAATCTTTAAAAAT
>ERR032221.298_IL9_4848:1:1:1206:4000/2
GGTTAAAAAAACCAACCCTCTCTTTTCCCCGCCGCCCCCCCCCCACCCACTTCCCCCACCCCCACCTCTTCGTACT
>ERR032221.304_IL9_4848:1:1:1207:6733/2
CGGGGACGGGGGGGAGCGGGGGTCTCTCTCCCCCCCACCCCACACCCCACTCCTCAACCTCACACCCAGCTACGGC
>ERR032221.311_IL9_4848:1:1:1208:14062/1
GCCGTTCCGAAGGGACGGGCGATTGCCGCCCATGCCCCCAGCCCGGAACAAAAGGAGGGGGGGCTCGANCCCCTAA
>ERR032221.325_IL9_4848:1:1:1210:7020/2
TGTGTGTGTGTGTGTGTATGTGTGTGTGTGTAGGGAGTTGATATTCCTGGTCCTTTTTTGATGAAAAATAGTAGTC
>ERR032221.348_IL9_4848:1:1:1215:19186/1
GCAGCTCCCTGCAGTCCACAGCTGGTCTCCTGGCACTGCTCCTACCCACGCAGATTTACTGCAACCAANCATCTGT
>ERR032221.350_IL9_4848:1:1:1215:4311/1
GTTGGTTTTGATCTGATAAATGCACGCATCCCCCCGGAGATTTTTTTTTATGTAAAAAATTTAATGTTNGATATAT
>ERR032221.360_IL9_4848:1:1:1216:4729/1
GTGAGGGCCACGAGGTGGCAAAGAGCTAAGGCCCTGAGTTCTGACAGCGAGGAACTGTTAAATGAAGCNAAGATGA
>ERR032221.361_IL9_4848:1:1:1217:1131/2
ATAANANACNTNGTAAGTNAGTCTCTTGATTCCAACCTCGAGTGAGNACGNANGGTNNNAGANCAGACCGTTGGNT
>ERR032221.395_IL9_4848:1:1:1224:16098/2
GTAATCGTGCTCCTTCTCCAAGACGCTCATGGCATCACACTTTGTGCACACCACCCAAATCTGATCAAATCAGTGG
>ERR032221.397_IL9_4848:1:1:1225:13501/2
GTTTTTTCCCCCCTGTCCCCGTGGCTCCCAGATTTACCAACGATGGAGCGGGGCGCAGGGGGGGCGGAGGGGCACG
>ERR032221.415_IL9_4848:1:1:1228:2827/2
CCCAGATCTGCACCCGCGGCGGGCCCCCCCCGGGCCCCCCCCCCGGGCTCCCAGGGCCCCCCCGCGGGGCCCCCGC
>ERR032221.418_IL9_4848:1:1:1228:5305/1
CCCTGACTTTAGCAAAAAAAAAAAAAAAAACAGTTTTTAACTGGGCTTTTTAAATTCTCCCCTAATTTNATGAAAA
>ERR032221.433_IL9_4848:1:1:1230:4158/1
TGAGACAAGCATATGCTACTGGCAGGATCAACCAGGTCCTCTGCAAAAGCAACAAGTACTCTTAACTGNTGAGCCA
>ERR032221.434_IL9_4848:1:1:1230:4110/1
TTTCTGACACCTCCTGCTTAAAACCCAATAAAATTAACGTGCCCTCCCTTCCCAAAACCCACACAGAANAACTTAA
>ERR032221.441_IL9_4848:1:1:1233:13648/2
CCCCCCGCCGCTTCCGCCCAGCGGCCAGCGCCGCAGCGCGCCGCGCCCGCCCGGCGCCCTCCCCCCCCCCCCCCCA
>ERR032221.444_IL9_4848:1:1:1233:8316/2
GCTGGAGTTTGCTTGCTGTGTGTGTGTTGTAAACATCAAGATTGTCTTTGAGTAATTGTTGGGTTTTTTTTCGGTT
>ERR032221.454_IL9_4848:1:1:1235:9698/2
AGAAGCCTCGGGAGGATGTTTCACATGGATATTGACTATGTTGGGGCAATCATCACTTTGACCCTTTATCAAGATG
>ERR032221.485_IL9_4848:1:1:1240:18811/1
TAAGGGAGTGTGGCATGGTTTGTGTGTGTGTGTGTGAGGCTGGGTTGATGTGATGTGCCCTGCACATCNCACATTC
>ERR032221.490_IL9_4848:1:1:1242:15876/2
CTCGGCTTACCTATTGATCACCCACAGGAGATTGAAGTTGATGGTGCTGAGTATCTCTGTACTGGCCGTTGGGGGG
>ERR032221.505_IL9_4848:1:1:1245:16347/1
TCATGTCTCTTCACCGTGCCAGACTAAGAACCTAGAACCCCGCTGGAAAGGACCCTACACCGTCCTGCNGACCACC
>ERR032221.530_IL9_4848:1:1:1251:19614/1
CCGCGAGGCGAGTCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCGGCCCGAAGCGTTTACTTTTNAAAAATT
>ERR032221.534_IL9_4848:1:1:1251:18498/2
CTTCACAACAGTGTAGAAGTAAGGGTGCTCCATGGCTTCACCCACGTCTCCCCTCACAGCTCTCAACTATGTGAAT
>ERR032221.544_IL9_4848:1:1:1253:3782/1
CTCGGCTAGGCATCCTGTCAGCAATGTGACCAACAGCACGGCCCAGGCAAATGAGGCAGAGGGACTCTNCTCAGCC
>ERR032221.547_IL9_4848:1:1:1254:18263/1
TTTATCACACAGTAGATTCCATCCCACGGGGAGTGTAAACATAGGGGACAGAATCAGGTATTGGCGTTNCTCCACG
>ERR032221.549_IL9_4848:1:1:1254:17106/2
CGCGTAACTAGTTAGCATGCCAGAGTCTCCTTCAGTTTTGCATCTGAAACAACAAAAACCACTCTATTCCCTCCCC
>ERR032221.550_IL9_4848:1:1:1254:10912/1
GGATTTCTGCTGGTGGATGGCCACACCCTGTAGCGACCTCACTGTATATAGCATGAGACTCCAGGTAGNTCCATGA
>ERR032221.585_IL9_4848:1:1:1261:13100/1
AAATAATCGGATCCAGAAAAAAACAAAGCAACGCCAACAACAAAAACCCAAAACACCTCATTGGCTAANACAAAAG
>ERR032221.594_IL9_4848:1:1:1263:20918/1
TTCCCTCCTCCCTTTACATGACNGCCAGGGATCCGACTCCAAGAAGTGGTCATCTGAGCTTTACAGATNTGCCATG
>ERR032221.606_IL9_4848:1:1:1266:2622/2
GGATCATCTGTGAAGAGTGCGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCC