Compare two protein sequences using Blast on Biopython without making a file for each sequence

Hi guys,

I have been trying to do this, but I've got stuck.

I know how to blast two sequences using biopython and the standalone Blast, but I make files in order to blast them.

I think making files increase run time a lot, so I would like to reduce it and my main idea is to avoid making files.

I have read about piping it, for example, straight with the command bl2seq and even in biopython, but I don't know, do pipe makes a temporary files?

Thanks in advance for your suggestions