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Blast "Max matches in a query range" option

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Hi Everyone, This is the first time I'm going to try such a web site to see if it works or not, hope it works!

So I have a few questions regarding blast, I appreciate it if you can help me,

  1. I see that blast has an evalue threshold option, is there a similar thing for percent identity? In other words is it possible to make it report only the hits with pident above a given threshold?

  2. I see that in online version: there is an option "Max matches in a query range" how can I use the same thing in stand alone version? and what should I put in as the value for this key?

  3. What is this option in standalone blast ? "-seg Filter query sequence with SEG (Format: 'yes', 'window locut hicut', or 'no' to disable) "

I appreciate your help,


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