Hello
I have some doubts on my analysis of an 454 EST s assembly of data that come from 3 different datasets. Each dataset come from the same organism, but in different conditions (resistance to fungi).
I've assembly them separated (I've tried the all together approach to, but for that my question doesn't apply) and I have blast them all. after that I had apply blast2go on blast results. How can I discover what is important for the conditions on test?
I have tried that approach:
- compare the blast best hits from each dataset with each other and said that, if a contig have the same best blast hit than other, it is the same thing
- that way, i define a group of reads that exists in all conditions an others that exist only on some conditions
- i made the blast2go enrichments analyses for this group of sequences, but because blast2go doesn't use only the best blast hit, the sequences that I had assume to be the same don't have the same GO terms
Any suggestions on how to continue?
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Ngs Multi-Dataset Analysis
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