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How to understand which DNA strand is (5' to 3' ot 3' to 5') in the result of blast ?

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Hi!

if a have such a result from a blast run, how can i understand if its in the 5' or 3' side of the DNA or protein strand ?

> gnl|SRA|SRR456295.4505.2 FIHSSUW02H237H.2 length=250
Length=250

 Score =   129 bits (324),  Expect = 1e-33, Method: Composition-based stats.
 Identities = 61/78 (78%), Positives = 68/78 (87%), Gaps = 0/78 (0%)
 Frame = +1

Query  203  RRMTLLLGPPGSGKTTLLLALAGRLNKDLKFSGQVTYNGHQMDEFVPQRTAAYISQHDLH  262
            R   L  GPP SGKTTLL+ALAG L K+LK SG+VTYNGH++ EFVP+RTAAYISQ+DLH
Sbjct  16   REWPLRPGPPSSGKTTLLMALAGTLAKELKSSGKVTYNGHELHEFVPERTAAYISQNDLH  195

Query  263  IGEMTVRETLAFSARCQG  280
            IGEMTVRETLAFSARCQG
Sbjct  196  IGEMTVRETLAFSARCQG  249

 

Thank you


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